Cucumber (Cucumis sativus) production is negatively affected by Zucchini yellow mosaic virus (ZYMV). Three sources of ZYMV resistance have been commercially deployed and all three resistances are conditioned by a single recessive gene.

A vacuolar protein sorting–associated protein 4-like (VPS4-like) gene has been proposed as a candidate for ZYMV resistance from cucumber line A192-18. We analyzed the genomic region across the VPS4-like gene for three independent sources of ZYMV resistance in cucumber (A192-18, Dina-1, and TMG-1) and identified three haplotypes across the coding region and considerable variation in the introns.

However, the haplotypes in the coding regions of the VPS4-like gene of A192-18, Dina-1, and TMG-1 encode the same protein sequence, revealing the genetic uniformity for ZYMV resistance from diverse germplasm sources.

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