Tomato Brown Rugose Fruit Virus (ToBRFV) is a particularly nasty virus that greatly impacts tomato yields. It spreads globally and can evade detection. Researchers found ToBRFV in wastewater, showing it's widespread in Ontario, Canada. So, they created a method to sequence ToBRFV genomes in wastewater, recovering 95.7% of the genome. To do that, they used existing resources for this and adapted a tool to estimate ToBRFV abundance.
Nissimov, Jozef. (2024). A Novel Tiled Amplicon Sequencing Assay Targeting the Tomato Brown Rugose Fruit Virus (ToBRFV) Genome Reveals Widespread Distribution in Municipal Wastewater Treatment Systems in the Province of Ontario, Canada. Viruses. 10.3390/v16030460.