Genetic key to salt-tolerance discovered in tilapia fish
To better understand such environmental flexibility, researchers at the University of California, Davis, have now identified short DNA segments in tilapia that influence the expression of the genes that regulate the fish’s internal body chemistry in response to salinity stress.
Additionally, the researchers developed an assay to identify similar regulatory DNA segments in the genomes of other fish species.
Studying Mozambique tilapia, the researchers found that short DNA segments enhance expression of genes that regulate the fish's internal body chemistry in response to salinity stress. (Photo by Greg Hume/Creative Commons)
“This work represents a critical milestone in our efforts to understand how highly stress-tolerant fish convert environmental signals and cues into very beneficial biochemical and physiological outcomes that enable them to adapt to an extremely wide salinity range that is deadly for most species,” said evolutionary biochemist and senior author Dietmar Kueltz.
“If we know these mechanisms, then we can target them in situations when fish would benefit from enhanced stress tolerance, such as in aquaculture and for conservation purposes,” said Kueltz.
Climate change and water salinity
Unlike humans and other land animals, fish and other aquatic animals are in a constant battle to maintain a balance between the water within their bodies and the water in which they live — a process known as osmoregulation. Salt plays a key role in this balancing act. If there is too much or too little salt in the surrounding water, cell membranes, tissues and organs are damaged, and the fish or animal dies, unless it compensates for the difference.
New developments
In the newly published study, the researchers studied cells from the Mozambique tilapia, one of four tilapia species that readily interbreed, producing hybrids that are used worldwide in aquaculture operations. Growing rapidly, these tilapia hybrids are easy to raise and have a high tolerance for salinity stress.
The researchers identified five DNA sequences, each containing a common segment that they named OSRE1, as being enhancers of the osmoregulation and salinity-response processes.
They also laid the groundwork for manipulating the OSRE1 enhancers, paving the way for future targeted studies aimed at identifying gene regulatory networks that confer salinity responsiveness to fish.
Source: UC Davis