Announcements

Job offersmore »



Tweeting Growers

Top 5 - yesterday

Top 5 - last week

Top 5 - last month

Exchange ratesmore »




New DNA test available for fast Xylella fastidiosa detection

After the release of the Clavibacter (Cmm) AmplifyRP detection test, Agdia commercializes a rapid and user-friendly DNA test kit, based on their AmplifyRP XRT+ platform, for detection of Xylella fastidiosa (Xf).

Xylella fastidiosa (Xf) is the causal agent of multiple crop diseases. Main crops affected by Xf are grape (Pierce's disease), citrus (citrus variegated chlorosis or CVC), almond (leaf scorch disease) and olives (quick decline syndrome).



Severe Xf infections can decimate entire crops and cause enormous financial losses to growers and the bacterium can also be a major threat for forest trees.
Historically, Xf was a problem in the American continent but since a few years outbreaks have been reported in Italy (with more than a million olive trees infected), France and more recently Spain.

The AmplifyRP XRT + test developed by Agdia can help to fight this disease by providing a rapid detection. This test is based on the isothermal amplification technique RPA (Recombinase Polymerase Amplification). RPA technology enables rapid amplification of nucleic acids (DNA or RNA) directly on crude sample extracts at a single operating temperature of 39C. This method has proved its reliability for many tests available on the market. It provides the same level of sensitivity and specificity as PCR laboratory methods. Results are obtained in less than 30 minutes, including sample preparation time. In addition, Agdia's Xylella fastidiosa kit can be used in the field to shorten test times to take the necessary sanitary measures as quickly as possible.



Agdia currently offers two formats of the AmplifyRP XRT + Xylella kit. For the first one, the target DNA is amplified at a constant temperature of 39C directly from a crude extract sample. The amplified product is then inserted into a detection chamber (photo n1) which allows a simple reading of the results on strips: positive or negative (like a pregnancy test).

For the second format, the user can carry out several tests at the same time thanks to a fluorometer (photo n 2) which will measure the fluorescence level of the amplified product and thus determine whether it is positive or negative for Xylella. The fluorometer reader is portable and battery powered.



For both methods, the complete test time is less than 30 minutes. The use of this kit does not require any special technical skills.

The goal of Agdia is to allow anyone to perform a simple and rapid test allowing to take quick decisions and helping the fight against this major pathogen.

For more information:
Agdia-Biofords
+33 (0) 1 60 78 81 64
info@agdia-biofords.com
www.agdia-biofords.com

Publication date: 3/9/2017

 


 

Other news in this sector:

10/17/2017 WUR: High levels of glyphosate in European agricultural soil
10/17/2017 Koppert and King Saud University sign research agreement
10/16/2017 NatureFresh Farms develops unconventional pest management methods
10/16/2017 Spain: Biobest recommends Nutrimite in sweet pepper
10/16/2017 NZ potato & tomato growers relieved at the release of pest eating wasp
10/16/2017 Protein restricts sap uptake by aphids
10/12/2017 Good results with PepMV vaccine in European countries
10/12/2017 Farmers dont want pesticides, but in the meantime, they have a business to run
10/11/2017 US (NY): Venerate XC Bioinsecticide now available in New York
10/10/2017 New Zealand: New biocontrol released to control Tomato Potato Psyllid
10/9/2017 Russell IPM is launching Optiroll Super plus in the USA
10/9/2017 US: Elumin fungicide now registered for downy mildew in cucurbits
10/9/2017 IAEA inaugurates new laboratory to help countries fight insect pests
10/6/2017 US (GA): Researchers search for management solutions for whiteflies
10/6/2017 Honey tests reveal global contamination by bee-harming pesticides
10/6/2017 Dominican Republic addressing prevalence of thrips among veg exports
10/5/2017 Seven biocontrol products registered in Saudi Arabia
10/5/2017 Predatory insect may help with whitefly control
10/4/2017 New lab to boost UN efforts to keep harmful insects at bay
10/4/2017 Whitefly: Whats your (bio)type? Know as you grow!