Announcements

Job offersmore »





Tweeting Growers

Top 5 - yesterday

Top 5 - last week

Top 5 - last month

Exchange ratesmore »




New DNA test available for fast Xylella fastidiosa detection

After the release of the Clavibacter (Cmm) AmplifyRP detection test, Agdia commercializes a rapid and user-friendly DNA test kit, based on their AmplifyRP XRT+ platform, for detection of Xylella fastidiosa (Xf).

Xylella fastidiosa (Xf) is the causal agent of multiple crop diseases. Main crops affected by Xf are grape (Pierce's disease), citrus (citrus variegated chlorosis or CVC), almond (leaf scorch disease) and olives (quick decline syndrome).



Severe Xf infections can decimate entire crops and cause enormous financial losses to growers and the bacterium can also be a major threat for forest trees.
Historically, Xf was a problem in the American continent but since a few years outbreaks have been reported in Italy (with more than a million olive trees infected), France and more recently Spain.

The AmplifyRP XRT + test developed by Agdia can help to fight this disease by providing a rapid detection. This test is based on the isothermal amplification technique RPA (Recombinase Polymerase Amplification). RPA technology enables rapid amplification of nucleic acids (DNA or RNA) directly on crude sample extracts at a single operating temperature of 39°C. This method has proved its reliability for many tests available on the market. It provides the same level of sensitivity and specificity as PCR laboratory methods. Results are obtained in less than 30 minutes, including sample preparation time. In addition, Agdia's Xylella fastidiosa kit can be used in the field to shorten test times to take the necessary sanitary measures as quickly as possible.



Agdia currently offers two formats of the AmplifyRP XRT + Xylella kit. For the first one, the target DNA is amplified at a constant temperature of 39°C directly from a crude extract sample. The amplified product is then inserted into a detection chamber (photo n°1) which allows a simple reading of the results on strips: positive or negative (like a pregnancy test).

For the second format, the user can carry out several tests at the same time thanks to a fluorometer (photo n ° 2) which will measure the fluorescence level of the amplified product and thus determine whether it is positive or negative for Xylella. The fluorometer reader is portable and battery powered.



For both methods, the complete test time is less than 30 minutes. The use of this kit does not require any special technical skills.

The goal of Agdia is to allow anyone to perform a simple and rapid test allowing to take quick decisions and helping the fight against this major pathogen.

For more information:
Agdia-Biofords
+33 (0) 1 60 78 81 64
info@agdia-biofords.com
www.agdia-biofords.com

Publication date: 3/9/2017

 


 

Other news in this sector:

6/23/2017 LSU AgCenter researchers receive USDA grant to study pollinator health
6/23/2017 Probiotics may help bees survive crop protection exposure
6/23/2017 What is a biopesticide?
6/23/2017 Costa Rica reduces agrochemicals use
6/23/2017 Which Biopesticides are available for use in U.S. greenhouses?
6/23/2017 Citrus greening spreads to Alabama, US
6/22/2017 US: Expanded approval for Abamex insecticide
6/22/2017 Koppert improves biological control of thrips
6/22/2017 Suppressing phytophthora root rot in northern highbush blueberry
6/21/2017 USDA gets serious about saving bees
6/21/2017 "Diagnostics and knowledge of bacteria needed to prevent spread of diseases"
6/20/2017 WUR research digs into management of greenhouse air fungi
6/20/2017 Why does bee health matter?
6/20/2017 Belgian universities to develop agricultural biologicals
6/20/2017 New AHDB RB209 Nutrient Management app available from Apple Store
6/19/2017 Monsanto and Atomwise collaborate on AI crop protection
6/16/2017 Isagro USA to market Vestaron’s Spear-T
6/16/2017 Manage disease to help produce tomato fruit
6/16/2017 Effective pest control in tomato starts with effective monitoring
6/16/2017 "Biodiversity is the basis for Integrated Pest Management"