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Rwanda: Pepper viruses found in chilli peppersIn January 2016, 35 leaf dried samples of chilli pepper (Capsicum sp.) were submitted to Fera Science Ltd, from six districts in Rwanda: Ruhango, Nyanza, Rulindo, Kayonza, Nyagatare and Kirehe. The samples were sent in following the appearance of a suspected virus in the chilli pepper crops. Symptoms before drying included distorted leaves, stunting and mosaic (Fig. 1). Chilli pepper is an important horticultural crop in Rwanda; in 2017, 27 tonnes of chilli pepper were exported, mainly to the UK, the Netherlands and Belgium, worth approximately US $54,000 (National Agricultural Export Development Board, Rwanda).
Figure 1: Infected Capsicum plants showing stunting, distortion and mosaic (courtesy of Bellancile Uzayisenga).
Following a previous finding of an unknown potyvirus in a sample of Capsicum with the same symptoms from Rwanda in June 2015, the samples were tested by ELISA with generic potyvirus antisera from the Leibniz-Institut DSMZ (Braunschweig, Germany). A potyvirus was detected by ELISA in all the samples. To try to identify the potyvirus by sequencing, the samples were tested by PCR using the P9502 and CPUP potyvirus primers (van der Vlugt et al., 1999). However, the presence of a potyvirus could not be confirmed in this way. The samples were also tested by ELISA for known potyviruses found in Capsicum, including Chilli veinal mottle virus (DSMZ) and Potato virus Y (Bioreba, Reinach, Switzerland). These ELISA tests were negative. Therefore, one of the samples from the Ruhango region was screened using an Illumina MiSeq as described by Adams et al. (2014). Sequences for the following viruses were derived from the MiSeq run and added to GenBank: Pepper veinal mottle virus (PVMV, genus Potyvirus; GenBank Accession No. MG470801), Pepper yellows virus (PeYV, genus Polerovirus; MG470802) which is a newly described virus detected in pepper (Lotos et al., 2017), Cucumber mosaic virus (CMV, genus Cucumovirus; MG470798, MG470799 and MG470800) and a novel virus which is tentatively a member of the genus Enamovirus (MG470803). Real time PCR assays were designed (Table 1) to PVMV and PeYV and all the samples were tested using these assays. Both PVMV and PeYV were detected in samples from Ruhango, Nyanza and Nyagatare. However, PeYV but not PVMV, was detected in samples from Kayonza, Rulindo and Kirehe.
Following the sequencing results the samples were also tested by ELISA to confirm CMV infection (Agdia, Elkhart, Indiana, USA). CMV was detected in the sample tested by next generation sequencing (Ruhango region) and in samples from Nyanza and Rulindo, but not in the samples from Kayonza, Nyagatare and Kirehe.
This is the first confirmed report of PVMV and PeYV in Rwanda and the first detection of a novel enamovirus. Work is ongoing to try to characterise the enamovirus.
Source: New Disease Reports
Publication date: 2/1/2018
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