Announcements

Job offersmore »

Tweeting Growers

Top 5 - yesterday

Top 5 - last week

Top 5 - last month

Exchange ratesmore »




Hybrid purity testing of Brassica rapa using SSR marker technology

In a new study, thirteen Chinese cabbage (Brassica rapa) hybrid cultivars and 26 parental inbred lines were used as experimental materials to screen for primers producing hybrid and parental complementary bands and for primers with high polymorphism information contents and low genotype frequencies. A total of 18 pairs of core primers were designed to identify the purity of Chinese cabbage.

There was no significant difference in the purity percentage measured between different loci of the same strain. The fingerprint obtained by the amplification of each locus could be used to identify purity to obtain an authentic purity percentage. Curve mapping and significance analyses were conducted using the purity percentage of eight different seed samples and confirmed a sampling seed number of 96.

The results of the purity test were verified by comparison with the grow-out test (GOT) using molecular markers. In conclusion, the simple sequence repeat (SSR) detection system could be used for the rapid identification of the purity of the tested Chinese cabbage hybrids.

Access the full study at HortScience.

Publication date: 11/14/2017

 


 

Other news in this sector:

7/20/2018 Syngenta lettuce varieties, resistant to the new Bremia races BI: 34-35
7/20/2018 Plant breeder shares profits with growers in developing countries
7/20/2018 New company seeks to shake up plant breeding
7/20/2018 New Zealand: Retired researcher breeds out-of-season strawberries
7/19/2018 Ukraine: Tomato Field Day in Kamenka
7/18/2018 US: Sakata pledges support of the Seed Your Future movement
7/12/2018 Regulatory status of non-GMO plant innovations under current EU law
7/6/2018 CPVO sees technical challenges to EU PVP system
7/3/2018 Gene editing approach aims for broad disease resistance in food crops
7/2/2018 Italy: New tomato varieties for an increasingly demanding market
7/2/2018 EC ruling may offer opportunities for situation-specific safety analyses
7/2/2018 Chinese scientists spot selfish genetic element in plants
7/2/2018 US (CA): Lettuce breeder expands to include grocers
7/2/2018 US: $50,000 grant for novel gene editing approach
6/29/2018 Plants have unique lock to control expression of genes
6/28/2018 Tim March new business manager at Rijk Zwaan Australia
6/28/2018 Tomatoes with a Western-Asian background
6/26/2018 "The seed business is about trust"
6/18/2018 High attendance for Open House Days of Eminent Seeds
6/15/2018 ASTA announces 2018 seed industry award winners