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China: TLED improves challenging cultivation of tissue culture

The South China Normal University College of Life Sciences was founded in January 2002, as the successor to the Department of Biology. This institution has a long history and high level of social influence. Wang Xiaojing, a professor at the college, has 25 years of research experience in the use of light to regulate plant growth and development.



Professor Wang's team was faced with a new challenge: the cordyline fruticosa tissue culture seedlings of a local tissue culture enterprise had poor uniformity, with a high rate of mutation. The enterprise urgently needed to solve this problem. Due to the many successes of Philips horticultural LED lighting in the tissue culture field, the enterprise formed a three-way partnership with the College of Life Sciences and Philips. Professor Wang's research team began to work on the problem, and their goal was to find an LED formula that would improve the reproduction rate and tissue culture seedling uniformity of cordyline fruticosa cultures.



Philips horticultural experts provided Professor Wang's research team with versions V1 to V6 of their GreenPower TLED products for testing purposes. The team kept detailed records of the various environmental data for each tested version and worked with Philips to regularly analyze the differences between the results of different processing methods. Ultimately, the team selected the Philips GreenPower high-blue white light TLED product to use for cordyline fruticosa tissue cultures. These lights reduced the tissue culture seedling shoot mutation rate, increasing uniformity and the rate of propagation. This light source type also increased the dry matter accumulation and anthocyanins content for a certain variety of cordyline fruticosa tissue culture seedling. These findings were very satisfactory to the enterprise that commissioned the work.



Compared with fluorescent lighting, the use of Philips GreenPower high-blue white light TLED lighting further enhanced the quality of cordyline fruticosa tissue culture seedling growth. The shoot propagation rate increased by 31% and the shoot mutation rate decreased by 37%. For one cordyline fruticosa variety, the dry matter ratio increased by 10% and the chlorophyll content increased by 13%. The tissue culture seedlings became more robust.

In the three-way cooperation between Phillips, the college, and the tissue culture enterprise, professor Wang's team proved their strong research abilities. They effectively communicated and interacted with experts from Phillips and the technical staff of the tissue culture enterprise, timely obtained information on the first-line technical support needs of the production process, and conducted a research project with practical applications.

For more information
Philips Horticulture LED Solutions
Daniela Damoiseaux
Global MarCom Manager
High Tech Campus 45, Eindhoven
HTC 45, Ground Floor, PO Box 80020, 5600JM Eindhoven
T: +31 6 31 65 29 69

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